LifeCanvas Technologies @LifeCanvasTech
Revolutionizing bioimaging to elevate human health. Pioneering powerful end-to-end solutions for intact tissue #spatialbiology. lifecanvastech.com Cambridge, MA Joined June 2018-
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The intact mouse brain was labeled with NeuN using SmartBatch+ and imaged with #SmartSPIM at 3.6X magnification. The images were aligned to the Allen Mouse Brain Atlas using an automated, multi-stage registration pipeline. Sample courtesy of Ian Meng, University of New England.
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This mouse brain (5XFAD x BXD29) was stained using SmartBatch+ with Thioflavin S dye, which binds to Beta Amyloid plaques and Tau tangles. It was captured with #SmartSPIM at 3.6X magnification. Sample courtesy of David Ashbrook & Robert W. Williams, UTHSC; G. Allan Johnson, Duke.
The neuropeptide Orexin is produced by neurons in the hypothalamus and regulates sleep, appetite, and reward-seeking behaviors. Orexin blockers are being studied as a treatment for addiction and sleep disorders. Mouse brain labeled with SmartBatch+ and imaged with #SmartSPIM.
Beta Tubulin (red) labels type I spiral ganglion neurons in the mouse cochlea. NF-H (blue) labels the auditory nerve fibers, which carry information from the hair cells to the cochlear nucleus. Imaged with #SmartSPIM at 9X. Sample courtesy of Dr. Bohua Hu, UT Dallas.
Thy1-GFP transgenic mouse lines are used to generate stunning images of fine neuronal structures. Different Thy1 mouse lines express GFP across variable cell populations, hitting around 10% of cells in a particular group. Clear+ delipidation and #SmartSPIM imaging at 3.6X.
This intact mouse brain was labeled with DyLight 594 Tomato Lectin with SmartBatch+, resulting in uniform labeling of blood vessels throughout the brain. In this small block of brain cortex, hundreds of capillaries form a dense network to disperse nutrients and clear away waste.
GFAP labeling astrocytes in the corpus callosum and cortex in a mouse brain. The whole mouse brain was cleared with Clear+ delipidation buffer, labeled with SmartBatch+, and imaged with #SmartSPIM at 3.6X magnification. #tissueclearing #neuroscience #brainresearch
These Purkinje cells tagged with mOrange2 are showing off their distinctive fan shape and single plane orientation. Purkinje cells are found only in the cerebellar cortex and are involved in motor control. #SmartSPIM, 9X magnification. Sample courtesy of Michelle Antoine, NIH.
DeltaFosB expression in a mouse brain, labeled using LifeCanvas protocols and imaged with #SmartSPIM at 3.6X magnification. Sample courtesy of Joselyn Soto, UCLA.
LifeCanvas Technologies retweeted
Happy #3DThursday! Today we'll explore a SUB(!!)-volume of 500GB from a cleared, intact mouse brain from Jose Maldonado at @VanderbiltU . Here you can see how we render these enormous data on consumer grade GPUs. 🥽💻 🔎 Jose Maldonado, PhD 🔬 @LifeCanvasTech 🥽 @syGlassVR
New paper in #NatureBiotechnology: Introducing #CuRVE & #eFLASH, enabling rapid, uniform protein labeling in whole organs. Whole rodent brains labeled in just 1 day! Pushing #3DSpatialBiology forward: bit.ly/43c9AXO @MIT_Picower @MIT
#TissueClearingWorkshop2025 at #MiCroN @harvardmed. Attendees saw how #SmartBatch+, #Megatome & #MegaSPIM enable automated sectioning & high-speed imaging, providing reliable data for research. Thanks for having us! Contact us: lifecanvastech.com/contact @LlopisMontero
🔬 Live from the #TissueClearingWorkshop2025 at @harvardmed MicRoN Core! We wrapped up a great talk on tissue clearing, and we're showcasing #SmartBatch+ & #MegaSPIM and #Megatome in action! Huge thanks to @LlopisMontero & the MicRoN team for hosting!
Dr. @LlopisMontero director of the MicRoN Core at @harvardmed shares how their vision aligns with LifeCanvas to support cutting-edge science. The core has integrated #MegaSPIM and #SmartBatch+ for active tissue clearing and light sheet imaging. Learn more: bit.ly/4fRQPf5
#FluorescenceFriday: Stunning lizard (Anolis) embryo imaging with #MegaSPIM! Thanks, Frederic Zimmer & Paul Maier @MBLScience. Explore #MegaSPIM for unmatched volumetric imaging: lifecanvastech.com/products/megas…
#FluorescenceFriday | Today #MegaSPIM transforms #zebrafish biology! 3D video of an adult #zebrafish, with scales & bones autofluorescing under 488nm. Captured at @MBLScience by @amrevakiraheen & @carsten_wolff. Dive in: bit.ly/4bVonrf #Microscopy #Researchers
Dr. Andy Stone, Manager at @BrandeisU Light Microscopy Core, calls #SmartSPIM & #SmartBatch+ game-changers after a recent demo. Ease of use & reproducibility are just the beginning! Read more: bit.ly/3YZqF3m @andystone93 @BrandeisImaging #microscopy #Neuroscience
#FluorescenceFriday Check out this stunning video of GFAP-labeled astrocytes in a mouse brain, imaged with #SmartSPIM at 15X magnification! Contact us to learn more: lifecanvastech.com/contact-us/ #Brain #Neuroscience #Research
Human Brain Sectioning with #MegatomePremium - The hemisphere was sectioned at 4mm thickness and 30mm/min sectioning speed. Megatome allows precise, uniform tissue sectioning for a broad range of samples. Learn more:bit.ly/4hUnDGr #brainresearch #neuroscience #sectioning
#FluorescenceFriday: #SmartSPIM video from the Pattabiraman Lab (@Yale)! #GFP (green) & #TranssynapticTracing (red) reveal prefrontal cortex & thalamus connectivity in a neonatal mouse brain. Full video & details: bit.ly/3UUwKx0 Thanks to @kartikpattabir1 @_Dan_Doyle
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