Craig C. Mello @craigedit

We can do this people! Make planet earth great again! Love each other, question everything! Stop complaining and start Voting! Not every 4 years, every 2! 🤟 Rhode Island, USA Joined November 2010

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Craig C. Mello @craigedit

7 years ago

One more CRISPR story we just posted on Biorxiv. Adding 5' modifications to donors stimulates HDR and donor potency. Probably does so by preventing end joining reactions that reduce available donor in cells. Please pass along. biorxiv.org/cgi/content/sh…

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Craig C. Mello @craigedit

7 years ago

Don't forget to optimize your RNP concentration using a co-injection marker! It is toxic, you will still get short edits with ssODNs but you won't get long edits if the conc. is too high. Worms getting long dsDNA seem to be more sensitive to RNP dose.

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Craig C. Mello @craigedit

7 years ago

NEW CRISPR protocol: biorxiv.org/cgi/content/sh… Please share with your friends and let me know how the hybrid donors work for you! The idea is two PCR products one with homology arms, one without, mix melt and anneal. The hybrids improve efficiency of GFP-size inserts 5-10X

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Craig C. Mello @craigedit

8 years ago

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Craig C. Mello @craigedit

8 years ago

I meant 4098 miles in 2017.

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Craig C. Mello @craigedit

8 years ago

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Craig C. Mello @craigedit

8 years ago

#NewProfilePic

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Craig C. Mello @craigedit

8 years ago

The pew meeting in Tucson was fabulous! The guest speakers reminded us, our society relies on us! The talks were diverse, concise, and inspired! And although the skit was quite dumb, we had lots of fun. We're just bummed that Torsten retired. (Inspired by Anne Churchland)

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Craig C. Mello @craigedit

8 years ago

Just back from the best PEW meeting ever! Thanks to all the scholars, fellows, advisors and the whole PEW team especially Rebecca and Kara. Torsten, thanks for your vision! You will be back! I'm new to twitter so please add me to your contacts. Please stay in touch!!