Geoff Howe @GeoffTHowe
Postdoc @ Aguzzi Lab University of Zurich |SynBio PhD @ UCL | Virology, Gene Therapy, Functional Genetics Joined April 2020-
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Geoff Howe retweeted
In case you need the world's most powerful CRISPR ablation/activation/silencing reagents, our quadruple-guide vectors are now available at AddGene! addgene.org/Adriano_Aguzzi/
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Geoff Howe retweeted
CRISPR screening by AAV episome-sequencing (CrAAVe-seq): a scalable cell-type-specific in vivo platform uncovers neuronal essential genes: nature.com/articles/s4159…
Geoff Howe retweeted
In @NatRevGenet we outline strategies for in vivo pooled (single cell) CRISPR screens for genotype → phenotype mapping in animal models. This contains over a decade of hard earned knowledge by us and the field. Kudos to @AntonioSantinha & Alessio Strano. rdcu.be/eykft
Geoff Howe retweeted
I am super excited to announce that I will be starting my lab at the Department of Pharmacology of the University of Zurich in Switzerland next year!
Geoff Howe retweeted
This is a major success story in today's Science ALSP is a rare genetic condition – life expectancy is about 8 years after symptoms start, and there's no cure But a bone marrow transplant – in 8 patients – halted disease progression, maintained cognition and ability to walk
Geoff Howe retweeted
🧬Motifs are the new BLAST Folddisco scans 53M AlphaFold structures for catalytic, binding or allosteric patterns in seconds🔎 18× quicker, 3.5× smaller, hits in seconds🌌
Geoff Howe retweeted
CRISPR just scored its biggest win yet against Huntington's. The secret? A delivery system called RIDE that sneaks into neurons, makes its edit, then vanishes in 72 hours. Here's what happened 🧵👇
Geoff Howe retweeted
This is wild! Engineering E. coli bacteria to turn plastic waste into paracetamol (Tylenol) @NatureChemistry nature.com/articles/s4155… nature.com/articles/s4155…
Geoff Howe retweeted
Published today in @Nature, we describe an approach for single-molecule protein reading on @nanopore arrays. By utilizing ClpX unfoldase to ratchet proteins through a CsgG nanopore, we achieved single-amino-acid sensitivity. nature.com/articles/s4158…
Geoff Howe retweeted
Open Access UCL Research: An autonucleolytic suspension HEK293F host cell line for high-titre serum-free AAV5 and AAV9 production with reduced levels of DNA impurity discovery.ucl.ac.uk/id/eprint/1019…
Thanks to @MolTherapy for highlighting our paper published today!
Thanks to @MolTherapy for highlighting our paper published today!
Geoff Howe retweeted
📢 New paper alert: autonucleolytic suspension HEK line for high-titre serum-free #AAV5 and #AAV9 production with reduced DNA impurity. Nice one Geoff and colleagues at @ucl BioEng 👏🏼 @School_Pharmacy @uclResearch @_BSGCT @MolTherapy
📢 New paper alert: autonucleolytic suspension HEK line for high-titre serum-free #AAV5 and #AAV9 production with reduced DNA impurity. Nice one Geoff and colleagues at @ucl BioEng 👏🏼 @School_Pharmacy @uclResearch @_BSGCT @MolTherapy
Geoff Howe retweeted
So pleased to share our new paper in which @GeoffTHowe engineered an autonucleolytic HEK293F cell line, NuPro-1S, effecting a 60% reduction in high MW DNA impurities and, unexpectedly, increased vg / mL productivity for AAV5 and AAV9 #EngBio #genetherapy cell.com/molecular-ther…
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