Cristian Aparicio-Maldonado @_aparicioC
Biochemist and Virologist. Currently challenging myself with a Molecular Microbiology PhD. * Fan of multichannel pipettes Delft, Nederland Joined October 2014-
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Tomorrow 27th January, I will defend my PhD at TUDelft at 12 pm(GMT+1). My PhD research, entitled “DISARMing viral invaders of bacteria”, has been supervised by @StanBrouns and @NobregaFL Feel free to join the public defense lifestream: collegerama.tudelft.nl/mediasite/play… #phd #defense
This is a macrophage REPAIRING A BROKEN BLOOD VESSEL #MedTwitter #biology
Superb Postdoc position possibility with @RikkeMeyer5 at @iNANO_AarhusUni on investigation of the properties of secondary DNA structures in the extracellular matrix of bacterial biofilms international.au.dk/about/profile/…
Finishing a PhD requires a lot more graphic design ability than advertised, folks...
Filamentous #phages have a low burden on bacteria and compensate by providing services that aid in the formation of better #biofilms , delivering toxins and other factors that increase #virulence, or modifying their behavior to pro…lnkd.in/dRybs5Nm lnkd.in/d7EN4jW7
Programazo el viernes en Toledo!!! Gracias @BeM_Toledo por organizar esta jornada tan interesante!!
Programazo el viernes en Toledo!!! Gracias @BeM_Toledo por organizar esta jornada tan interesante!!
Congratualtions to my co-authors @_aparicioC @NobregaFL @StanBrouns @davidwtaylorjr. This was the first project I took on in the @davidwtaylorjr lab, and I'm really proud of the combination of cryo-EM, biochemistry and in vivo work we used to understand this fascinating system
Excited to share the final version of our DISARM story! How can bacterial confer incredibly broad antiphage immunity and yet avoid immunity? We think the answer may be due in part to DNA structural context and other mechanisms for cryptic specificity. nature.com/articles/s4146…
Great work by my postdoc @jpkbravo @TexasScience and @_aparicioC and @NobregaFL out in @NatureComms! First exciting collaboration with @StanBrouns. Structure of a complex that can broadly DISARM phage by grabbing and reading ssDNA tails! nature.com/articles/s4146…
This project was initiated by the magnificent @BalwinaK who carried out most of the work with @Potoc_A. Also many thanks to all other co-authors: @SumanthMutte @_aparicioC @StanBrouns, Simon Lindhoud and Jacques Vervoort. Thanks @NWO_Science and @ERC_Research for funding! 11/11
In eukaryotes, Argonautes (eAgos) are well known for their role in RNA-guided RNA interference. Prokaryotes encode Agos (pAgos) but lack other RNAi components. Instead, certain 'long' pAgos use DNA guides to target and cleave invading DNA such as plasmids and viruses. 2/11
Very pleased to share our ✨New Paper✨ about an epigenetic conflict between a restriction–modification system and a methylation-dependent nuclease. Want to know how that pans out? Continue reading here 🧵👇🏻 or head over to @NAR_Open for the full story: academic.oup.com/nar/advance-ar…
.#phagetherapy protocol approved: @PhageAustralia, a network of #phage researchers & clinicians, are excited 2 announce the approval of the STAMP phage therapy protocol, which regulates the process of phage therapy rather than a phage product specifically. buff.ly/3K7t17r
New bioRxiv feature: in-line figures for everyone! You can now print (or save as PDF) all articles with in-line figures, regardless of how the authors origiunally formatted them 1/n
A perfect mix of structure-guided biochemistry and in vivo experiments to determine how the DISARM immune system recognizes phage DNA!
A perfect mix of structure-guided biochemistry and in vivo experiments to determine how the DISARM immune system recognizes phage DNA!
@rafapinilla92 That’s what we proposed for DISARM - methyltransferase and nulease modules are clearly important yet DISARM still works if they’re not present. We speculated that other host methyltransferase and PLD nucleases can compensate, making those modules redundant
This is a first for me, a contaminant that turned my solid medium to liquid 😬🧫
Check out PhD student @ameliakschmidt's latest paper in collaboration with @theMaxwellLab out now @mbiojournal: A Filamentous Bacteriophage Protein Inhibits Type IV Pili to Prevent Superinfection of Pseudomonas aeruginosa doi.org/10.1128/mbio.0…
Preprint by @_aparicioC ( @tudelft, Netherlands) and colleagues: Class I DISARM provides anti #phage and anti-conjugation activity by unmethylated DNA recognition. buff.ly/3rpcFzl
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