Yun Kim @pairedbox
MD/PhD candidate & Vanier Scholar @uoftmedicine @sickkids, @the_rcm alumnus. Chromatin architecture, morphogenesis, & mechanobiology. He/him Toronto, Ontario Joined September 2019-
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Hypertranscription: the invisible hand in stem cell biology dlvr.it/TDBZQN
How do enhancers work over distances that sometimes exceed megabases? Excited to share our work led by @gracecbower where we uncover a unique sequence signature globally associated with long-range enhancer-promoter interactions in developing limb buds: biorxiv.org/content/10.110… 1/
Interested in epigenetics and the potential function of histone modifications? If so, Hua Wang in our lab has done some beautiful work in defining a role of H3K4me3!! Check out the paper - published today. nature.com/articles/s4158…
Absolute scaling of single-cell transcriptomes identifies pervasive hypertranscription in adult stem and progenitor cells dlvr.it/SgmPgb
Our paper on how matrix viscoelasticity controls tissue spatial and temporal organisation is now out in @NatureMaterials. @hseas @wyssinstitute @TheCrick @KCL_Physics @ERC_Research nature.com/articles/s4156…
Do specific locations in the nucleus preclude transcription? In a new paper led by @sofiquinodoz & Isabel Goronzy, we show that Pol II txn occurs throughout the nucleus, including locations thought to be inactive (B compartments & nucleoli). tinyurl.com/25ueavsp
Our work with Ming Guo @ MIT also just came out in Nature Physics. Here we looked at the effect of spatial curvature on a developing lung organoid. nature.com/articles/s4156…
We took on the challenge with @KateMiro1 to illustrate key aspects of #nuclear #mechanotransduction in a single #snapshot 👇Hope it’s useful! cell.com/cell/fulltext/…
Does an enhancer behave the same way in a different chromatin context? It seems it does not. Let's update our models for the appearance of new enhancers and the role of context in enhancer function. Encore: embryos with lots of blue and green hands. nature.com/articles/s4146…
Back to @MRSantosLab roots in SF for #ISSCR 2022. Great time presenting my work at the poster session yesterday. Stay tuned for a talk by @pairedbox Yun about hypertranscription tomorrow (Saturday) at 10:05 in the session "Principles of tissue and organ regeneration", room 2007.
For our first tweet, we share the lab's first research article. Grateful to have worked with Anna Erzberger @ErzbergerGroup on this project! cell.com/cell/fulltext/…
(1/6) our preprint biorxiv.org/content/10.110… is now out in @ScienceMagazine science.org/doi/10.1126/sc… Using super-res live-imaging we show that CTCF/cohesin loops are dynamic (~10-30 min median lifetime), rare (~3-6.5% looped fraction), and we fully parameterize a TAD.
Our paper where we address how epiblast and primitive endoderm mechanically segregate is out - congratulations to @gidayaka for her extraordinary work on this paper! @PDN_Cambridge @SCICambridge cell.com/cell/fulltext/…
📢#Hypertranscription preprint! Excellent exploration of global transcriptional shifts in adult organs probed w/ single-cell RNA-seq. Fantastic work, @pairedbox! The dynamic range of transcription is much broader than we thought. Stem cells are why. 1/3 biorxiv.org/content/10.110…
Cleavage stages: 2 days without much going on, as far as morphogenesis is concerned. Cells must be pretty quiet, right? Well, actually it depends on how fast you're imaging! Here is the latest preprint from the lab with @oozguc! biorxiv.org/content/10.110…
7 years in the making, and thanks to my amazing team @AlejandroAgCa @bernardoOldak @noashefi @Itaymaza & collaborators, our new paper is out. description will follow below later nature.com/articles/s4158…
When preparing a DNA sequencing library from a precious sample (e.g. single cell), @illumina Nextera loses 50% of DNA because it uses 2 transposons. Our method, META, solves this problem by using N = 20 transposons, reducing loss to 1/N = 5%. In @PNASNews: pnas.org/content/118/8/…
New Technology online now at Dev Cell: Inducible Stem-Cell-Derived Embryos Capture Mouse Morphogenetic Events In Vitro - zpr.io/HvtdA
Developmental clock and mechanism of de novo polarization started 7 years ago with the last 4 yrs critical. It took 2 yrs from the first submission. Each year @zhumeng123 expanded the work and @jake_cs_ added modelling. It feels so special. science.sciencemag.org/content/370/65…
Yun-Kyo Kim @pairedbox from the @MRSantosLab at LTRI presenting his investigation of hypertranscription (the global upregulation of txn) in different types of stem cells, at the resolution of a single-cell
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